Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Long term performance of polycaprolactone vascular grafts in a rat abdominal aorta replacement model
Sarra de Valence,Jean-Christophe Tille,Damiano Mugnai,Wojciech Mrówczyński,Robert Gurny,Michael Möller,Beat H. Walpoth +6 more
TL;DR: Issues with degradable vascular grafts that cannot be identified with short implantation times or in vitro studies are presented and should allow for better design of next generationascular grafts.
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Biological applications of microfluidic gradient devices
TL;DR: The principles and applications of microfluidic gradient generation in both 2D and 3D cellular microenvironments are discussed with emphasis on approaches to manipulate spatial and temporal distribution of signaling molecules.
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Tuning Supramolecular Rigidity of Peptide Fibers through Molecular Structure
TL;DR: Molecules that form supramolecular structures with the highest mechanical stiffness were found by circular dichroism to self-assemble into beta-sheets with the least amount of twisting and disorder, a result that is consistent with IR experiments.
Journal ArticleDOI
Transglutaminase Regulation of Cell Function
Richard L. Eckert,Mari T. Kaartinen,Maria Nurminskaya,Alexey M. Belkin,Gozde Colak,Gail V.W. Johnson,Kapil Mehta +6 more
TL;DR: The studies described herein begin to clarify the physiological roles of TGs in both normal biology and disease states and highlight the mechanism of action of these proteins with respect to their structure, impact on cell differentiation and survival, role in cancer development and progression.
Journal ArticleDOI
Engineering the regenerative microenvironment with biomaterials.
Jeffrey J. Rice,Mikaël M. Martino,Laura De Laporte,Federico Tortelli,Priscilla S. Briquez,Jeffrey A. Hubbell +5 more
TL;DR: Important aspects of the healing microenvironment, and how these features can be incorporated within innovative hydrogel scaffolds, are presented.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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