Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Book ChapterDOI
Cellular functions of tissue transglutaminase.
TL;DR: The complex biochemical activities and molecular interactions of TG2 are discussed in the context of diverse subcellular compartments and its wide ranging and cell type-specific biological functions and their regulation are evaluated.
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Actomyosin stiffens the vertebrate embryo during crucial stages of elongation and neural tube closure
TL;DR: Stiffness measurements from explants with reduced fibronectin fibril assembly or disrupted actomyOSin contractility suggest that it is the state of the actomyosin cell cortex rather than accumulating fibronECTin that controls tissue stiffness in early amphibian embryos.
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Artificial Stem Cell Niches
TL;DR: A perspective on how advanced materials technologies could be used to engineer and systematically analyze specific aspects of functional stem cells niches in a controlled fashion in vitro and to target stem cell Niches in vivo is provided.
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Brillouin microscopy: an emerging tool for mechanobiology
Robert Prevedel,Alba Diz-Muñoz,Giancarlo Ruocco,Giancarlo Ruocco,Giuseppe Antonacci,Giuseppe Antonacci +5 more
TL;DR: This Review discusses the principles, advantages and limitations of Brillouin microscopy, a non-invasive tool for measuring mechanical properties of biological samples in three dimensions, as well as its potential for gaining insights in biology.
Journal ArticleDOI
Tunable mechanics of peptide nanofiber gels.
TL;DR: The ability to modify how self- assembled fibrillar networks respond to deformations is important in developing self-assembled gels that can resist and recover from the large deformations that these gels encounter while serving as synthetic cell scaffolds in vivo.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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