Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Design of three‐dimensional biomimetic scaffolds
Shawn C. Owen,Molly S. Shoichet +1 more
TL;DR: Some of the most significant biological features of the ECM are described, and several engineering methods currently being implemented to design and tune synthetic scaffolds to mimic these features are described.
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A Review of 3D Printing Techniques and the Future in Biofabrication of Bioprinted Tissue
Satyajit Patra,Vanesa Young +1 more
TL;DR: Three promising methods of 3D printing emerged from their research: thermal inkjet printing with bioink, direct-write bioprinting, and organ printing using tissue spheroids are discussed.
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Novel 3D Culture Systems for Studies of Human Liver Function and Assessments of the Hepatotoxicity of Drugs and Drug Candidates.
Volker M. Lauschke,Delilah F. G. Hendriks,Catherine C. Bell,Tommy B. Andersson,Tommy B. Andersson,Magnus Ingelman-Sundberg +5 more
TL;DR: A review of the development of 3D liver models can be found in this article, where the authors highlight the need to develop more integrated coculture model systems to emulate immunotoxicities that arise due to complex interactions between hepatocytes and immune cells.
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Delivery of a Therapeutic Protein for Bone Regeneration from a Substrate Coated with Graphene Oxide
Wan Geun La,Saibom Park,Hee Hun Yoon,Gun-Jae Jeong,Tae-Jin Lee,Suk Ho Bhang,Jeong Yeon Han,Kookheon Char,Byung Soo Kim +8 more
TL;DR: Graphene oxide (GO) is an effective carrier for the controlled delivery of therapeutic proteins, such as BMP-2, which promotes osteointegration of orthopedic or dental Ti implants.
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Scaffold stiffness influences cell behavior: opportunities for skeletal tissue engineering
TL;DR: Current knowledge on the role of the scaffold stiffness in the regulation of cell behavior is summarized and how this knowledge can be incorporated in scaffold design which may provide new opportunities in the context of orthopedic tissue engineering is discussed.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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