Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation
Marco Costantini,Marco Costantini,Joanna Idaszek,Krisztina Szöke,Jakub Jaroszewicz,Mariella Dentini,Andrea Barbetta,Jan E. Brinchmann,Jan E. Brinchmann,Wojciech Święszkowski +9 more
TL;DR: The observed enhanced viability and chondrogenic differentiation of BM-MSCs, as well as high robustness and accuracy of the employed deposition method, make the presented approach a valid candidate for advanced engineering of cartilage tissue.
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Rescuing replication and osteogenesis of aged mesenchymal stem cells by exposure to a young extracellular matrix
Yun Sun,Weiping Li,Zhengding Lu,Richard Chen,Jian Ling,Qitao Ran,Robert L. Jilka,Xiao Dong Chen +7 more
TL;DR: It is concluded that aging negatively affects the formation of an ECM that normally preserves MSC function, and aged MSCs can be rejuvenated by culture on a young‐ECM.
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From Mechanical Force to RhoA Activation
TL;DR: This review focuses on the pathways by which mechanical force leads to RhoA regulation, especially when force is transmitted via cell adhesion molecules that mediate either cell-matrix or cell-cell interactions.
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Material stiffness influences the polarization state, function and migration mode of macrophages.
Rukmani Sridharan,Brenton Cavanagh,Andrew R. Cameron,Andrew R. Cameron,Daniel J. Kelly,Daniel J. Kelly,Fergal J. O'Brien,Fergal J. O'Brien +7 more
TL;DR: This study reports that THP-1 derived macrophages cultured on collagen-coated polyacrylamide gels of varying stiffness adapt their polarization state, functional roles and migration mode according to the stiffness of the underlying substrate and suggests that biomaterial stiffness is capable of directing macrophage behaviour independent of the biochemical cues being presented to them.
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Composite Hydrogels with Tunable Anisotropic Morphologies and Mechanical Properties
Mokit Chau,Bernd Kopera,Bernd Kopera,Vanessa R. Machado,Sabine Rosenfeldt,Laura M. Reyes,Katelyn Chan,Stephan Förster,Emily D. Cranston,Todd Hoare,Eugenia Kumacheva +10 more
TL;DR: In this article, a freeze-casting-based fabrication of structurally and mechanically anisotropic aerogels and hydrogels composed of hydrazone cross-linked poly(oligoethylene glycol methacrylate) (POEGMA) and cellulose nanocrystals (CNCs) was reported.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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