Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
More filters
Journal ArticleDOI
Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation
Joaquin Cortiella,Jean A. Niles,Andrea V. Cantú,Andrea Brettler,Anthony Pham,Gracie Vargas,Sean Winston,Jennifer M Wang,Shannon Walls,Joan E. Nichols +9 more
TL;DR: It is found that AC lung allowed for better retention of cells with more differentiation of mESCs into epithelial and endothelial lineages and highlights the critical role played by matrix or scaffold-associated cues in guiding ESC differentiation toward lung-specific lineages.
Journal ArticleDOI
An active biopolymer network controlled by molecular motors
Gijsje H. Koenderink,Zvonimir Dogic,Fumihiko Nakamura,Poul Martin Bendix,Fred C. MacKintosh,John H. Hartwig,Thomas P. Stossel,David A. Weitz +7 more
TL;DR: An active polymer network in which processive molecular motors control network elasticity is described and suggests that adherent cells exert mechanical control by operating in a nonlinear regime where cell stiffness is sensitive to changes in motor activity.
Journal ArticleDOI
Jekyll and Hyde: the role of the microenvironment on the progression of cancer.
Michael D. Allen,J. Louise Jones +1 more
TL;DR: It is becoming evident that characterizing the tumours microenvironment can provide important prognostic and predictive information about tumours, independent of the tumour cell phenotype, with a number of molecules already in clinical trials.
Journal ArticleDOI
Bone tissue regeneration: the role of scaffold geometry
TL;DR: Recent discoveries concerning the effects of geometrical features of porous scaffolds such as surface curvature, pore shape, and pore size on the cellular response and bone tissue regeneration process are reviewed and recommended for future research.
Journal ArticleDOI
Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels
William J. Hadden,Jennifer L. Young,Andrew W. Holle,Andrew W. Holle,Meg L. McFetridge,Du Yong Kim,Philip Wijesinghe,Hermes Taylor-Weiner,Jessica H. Wen,Andrew Lee,Karen Bieback,Ba-Ngu Vo,David D. Sampson,Brendan F. Kennedy,Joachim P. Spatz,Joachim P. Spatz,Adam J. Engler,Yu Suk Choi +17 more
TL;DR: A method of polymerization control in which the differential diffusion distance of unreacted cross-linker and monomer into a prepolymerized hydrogel sink results in a tunable stiffness gradient at the cell–matrix interface is developed, making it possible to pinpoint optimal stiffness values for a wide range of biological phenomena without the confounding effects of durotaxis.
References
More filters
Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
Related Papers (5)
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more