Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression.
Fei Liu,Justin D. Mih,Barry S. Shea,Alvin T. Kho,Alvin T. Kho,Asma S. Sharif,Andrew M. Tager,Daniel J. Tschumperlin +7 more
TL;DR: In response to tissue stiffening, fibroblasts increase production of extracellular matrix while decreasing production of matrix-degrading enzymes and the fibrosis inhibitor prostaglandin E2.
Journal ArticleDOI
Causes, consequences, and remedies for growth-induced solid stress in murine and human tumors
Triantafyllos Stylianopoulos,John D. Martin,John D. Martin,Vikash P. Chauhan,Saloni R. Jain,Saloni R. Jain,Benjamin Diop-Frimpong,Benjamin Diop-Frimpong,Nabeel Bardeesy,Barbara L. Smith,Cristina R. Ferrone,Francis J. Hornicek,Yves Boucher,Lance L. Munn,Rakesh K. Jain +14 more
TL;DR: This work shows that therapeutic depletion of carcinoma-associated fibroblasts with an inhibitor of the sonic hedgehog pathway reduces solid stress, decompresses blood and lymphatic vessels, and increases perfusion, and can serve as a rapid screen for stress-reducing and perfusion-enhancing drugs.
Journal ArticleDOI
Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low-Viscosity Bioink.
Cristina Colosi,Cristina Colosi,Cristina Colosi,Su Ryon Shin,Su Ryon Shin,Su Ryon Shin,Vijayan Manoharan,Vijayan Manoharan,Solange Massa,Solange Massa,Solange Massa,Marco Costantini,Andrea Barbetta,Mehmet R. Dokmeci,Mehmet R. Dokmeci,Mehmet R. Dokmeci,Mariella Dentini,Ali Khademhosseini +17 more
TL;DR: A novel bioink and a dispensing technique for 3D tissue-engineering applications are presented, which incorporates a coaxial extrusion needle using a low-viscosity cell-laden bioink to produce highly defined 3D biostructures.
Journal ArticleDOI
Engineering biocompatible implant surfaces
TL;DR: The authors in this paper reviewed the interdisciplinary field of biocompatible implant surfaces from the viewpoint of materials science, biochemistry and cell biology, and provided an overview on basic information about bulk and surface properties of implant surfaces.
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2D and 3D cell cultures - a comparison of different types of cancer cell cultures.
Marta Kapałczyńska,Tomasz Kolenda,Weronika Przybyła,Maria Małgorzata Zajączkowska,Anna Teresiak,Violetta Filas,Matthew Ibbs,Renata Bliźniak,Łukasz Łuczewski,Katarzyna Lamperska +9 more
TL;DR: 2D and 3D cultures in vitro as well as different versions of3D cultures are compared to allow for a better understanding of cancer biology and facilitate the study of biomarkers and targeting therapies.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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