Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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YAP/TAZ functions and their regulation at a glance
TL;DR: A hitchhiker's guide to the rapidly expanding YAP/TAZ regulatory network, centered on the Hippo pathway and on mechanosensing of the physical properties of the cell microenvironment.
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Mechanical regulation of mesenchymal stem cell differentiation
TL;DR: The strides that have been made in elucidating how MSCs sense and respond to their mechanical environment are illustrated, and areas where further research is needed are identified.
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Injectable scaffolds: Preparation and application in dental and craniofacial regeneration.
TL;DR: This article provides a comprehensive review of the injectable scaffolds currently being investigated for dental and craniofacial tissue regeneration, including natural, synthetic, and composite biomaterials.
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Keratins as the main component for the mechanical integrity of keratinocytes
Lena Ramms,Gloria Fabris,Reinhard Windoffer,Nicole Schwarz,Ronald Springer,Chen Zhou,Jaroslav Lazar,Simone Stiefel,Nils Hersch,Uwe Schnakenberg,Thomas M. Magin,Rudolf E. Leube,Rudolf Merkel,Bernd Hoffmann +13 more
TL;DR: The used keratin KO cells lacking complete keratin gene clusters to result in total loss of keratin filaments are used to demonstrate the importance of keratins for mechanical resilience of Keratinocytes and indicate that expression of a single keratin pair is sufficient for almost complete reconstitution of their mechanical properties.
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Modulation of alignment, elongation and contraction of cardiomyocytes through a combination of nanotopography and rigidity of substrates
TL;DR: The distribution of intracellular structural proteins showed that the surface topography and substrate stiffness regulated the organization of the actin cytoskeleton and focal adhesion complexes, and consequently the contractile behavior of the cardiomyocytes.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
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Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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