Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Journal ArticleDOI
Extracellular Matrix Promotes Highly Efficient Cardiac Differentiation of Human Pluripotent Stem Cells: The Matrix Sandwich Method
Jianhua Zhang,Matthew Klos,Gisela F. Wilson,Amanda M. Herman,Xiaojun Lian,Kunil K. Raval,Matthew R Barron,Luqia Hou,Andrew G. Soerens,Junying Yu,Sean P. Palecek,Gary E. Lyons,James A. Thomson,Todd J. Herron,José Jalife,Timothy J. Kamp +15 more
TL;DR: In this article, the extracellular matrix is used to promote epithelial-mesenchymal transition of human PSCs and complemented growth factor signaling to enable robust cardiac differentiation.
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Antibacterial activity and increased bone marrow stem cell functions of Zn-incorporated TiO2 coatings on titanium
TL;DR: In this work, zinc was incorporated into TiO2 coatings on titanium by plasma electrolytic oxidation to obtain the implant with good bacterial inhibition ability and bone-formability, and excellent antibacterial activity and biocompatibility are promising candidates for orthopedic and dental implants.
Journal ArticleDOI
Mechanical signals as anabolic agents in bone
TL;DR: Mechanical targeting of the bone marrow stem-cell pool might represent a novel, drug-free means of slowing the age-related decline of the musculoskeletal system.
Journal ArticleDOI
Hypoxia-inducible Factor 1 (HIF-1) Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts
TL;DR: It is demonstrated that hypoxia-inducible factor 1 (HIF-1) is a critical regulator of ECM remodeling by fibroblasts under hypoxic conditions, which induces changes in breast cancer cell morphology, adhesion, and motility that promote invasion and metastasis.
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Artificial niche microarrays for probing single stem cell fate in high throughput
TL;DR: A microengineered platform comprised of soft hydrogel microwell arrays with modular stiffness in which individual microwells can be functionalized with combinations of proteins spotted by robotic technology is reported, showing the effect of key microenvironmental perturbations on the fate of any stem cell type in single cells and in high throughput.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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