Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Adult cell therapy for brain neuronal damages and the role of tissue engineering.
Gaëtan J.-R. Delcroix,Paul C. Schiller,Paul C. Schiller,Jean-Pierre Benoit,Jean-Pierre Benoit,Claudia N. Montero-Menei,Claudia N. Montero-Menei +6 more
TL;DR: The use of adult neural stem cells, adrenal chromaffin cells and retinal pigment epithelium cells for brain therapy, with a special emphasis on mesenchymal stromal cells are described.
Journal ArticleDOI
Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices
Haguy Wolfenson,Giovanni Meacci,Shuaimin Liu,Matthew R. Stachowiak,Thomas Iskratsch,Saba Ghassemi,Pere Roca-Cusachs,Ben O'Shaughnessy,James Hone,Michael P. Sheetz,Michael P. Sheetz +10 more
TL;DR: High temporal- and spatial-resolution tracking of contractile forces by plating cells on sub-micrometre elastomeric pillars finds that actomyosin-based sarcomere-like contractile units (CUs) simultaneously moved opposing pillars in net steps of ∼2.5 nm, independent of rigidity, which concludes that tropomyOSin 2.1 acts as a suppressor of growth on soft matrices by supporting proper rigidity sensing.
Journal ArticleDOI
Fine-tuning the degree of stem cell polarization and alignment on ordered arrays of high-aspect-ratio nanopillars.
TL;DR: The demonstrated ability to support various morphogenetic trends in stem cells by simply tuning the geometry of the NP substrates provides a stepping-stone for the future design of scaffolds where cellular morphology and alignment are crucial.
Journal ArticleDOI
Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
Laurie B. Hazeltine,Chelsey S. Simmons,Max R. Salick,Xiaojun Lian,Mehmet G. Badur,Wenqing Han,Stephanie M. Delgado,Tetsuro Wakatsuki,Wendy C. Crone,Beth L. Pruitt,Sean P. Palecek +10 more
TL;DR: It is demonstrated that hPSC-derived cardiomyocyte contractility responded appropriately to isoprenaline and remained stable in culture over a period of 2 months, which motivates their use in further applications such as drug evaluation and cardiac therapies.
Journal ArticleDOI
Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus
Huan Wang,Sarah M. Haeger,April M. Kloxin,Leslie A. Leinwand,Kristi S. Anseth,Kristi S. Anseth +5 more
TL;DR: Lower substrate modulus can serve as a cue to down-regulate the valvular myofibroblast phenotype resulting in a predominantly quiescent fibroblast population in vitro, providing insight in designing hydrogel substrates with physiologically relevant stiffness to dynamically redirect cell fate in vitro.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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