Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Graphene-based materials for tissue engineering.
Su Ryon Shin,Su Ryon Shin,Yi-Chen Li,Hae Lin Jang,Parastoo Khoshakhlagh,Mohsen Akbari,Amir Nasajpour,Yu Shrike Zhang,Ali Tamayol,Ali Khademhosseini +9 more
TL;DR: Graphene and its chemical derivatives have been a pivotal new class of nanomaterials and a model system for quantum behavior and the opportunities in the usage of graphene-based materials for clinical applications are outlined.
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Li Tang,Xujuan Yang,Qian Yin,Kaimin Cai,Hua Wang,Isthier Chaudhury,Catherine Yao,Qin Zhou,Mincheol Kwon,James A. Hartman,Iwona T. Dobrucki,Lawrence W. Dobrucki,Luke B. Borst,Stéphane Lezmi,William G. Helferich,Andrew L. Ferguson,Timothy M. Fan,Jianjun Cheng +17 more
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Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells
Ying Mei,Krishanu Saha,Said R. Bogatyrev,Jing Yang,Andrew L. Hook,Z. Ilke Kalcioglu,Seung Woo Cho,Maisam Mitalipova,Neena Pyzocha,Fredrick Rojas,Krystyn J. Van Vliet,Martyn C. Davies,Morgan R. Alexander,Robert Langer,Rudolf Jaenisch,Daniel G. Anderson +15 more
TL;DR: In this article, the authors identify the optimal substrates within a range of different polymeric surfaces to support the growth and self-renewal of human embryonic stem cells from fully dissociated single cells.
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Engineered Microenvironments for Controlled Stem Cell Differentiation
TL;DR: The premise is that to unlock the full potential of stem cells, at least some aspects of the dynamic three-dimensional environments that are associated with their renewal, differentiation, and assembly in native tissues need to be reconstructed.
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Evidence of a large-scale mechanosensing mechanism for cellular adaptation to substrate stiffness
Léa Trichet,Jimmy Le Digabel,Rhoda J. Hawkins,Sri Ram Krishna Vedula,Mukund Gupta,Claire Ribrault,Pascal Hersen,Raphaël Voituriez,Benoit Ladoux +8 more
TL;DR: It is shown that large-scale mechanosensing leads to an adaptative response of cell migration to stiffness gradients, and not only that cells migrate preferentially toward stiffer substrates, but also that this response is optimal in a narrow range of rigidities.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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