Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Tuning the Range of Polyacrylamide Gel Stiffness for Mechanobiology Applications
TL;DR: Gels are stiffer when polymerized at 4 °C compared to room temperature, indicating a complex relationship between gel structure, elasticity, and network formation, and this work uses Atomic Force Microscopy and force-distance curves to derive the elastic modulus of polyacrylamide hydrogels.
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Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function.
Megan L. McCain,Kevin Kit Parker +1 more
TL;DR: In both of these conditions, the cytoskeleton plays a pivotal role in both sensing mechanical stress and mediating structural remodeling and functional responses within the myocyte.
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Electrospun Nanofibers as Scaffolds for Skin Tissue Engineering
TL;DR: Electrospun polymeric nanofibrous scaffolds possess various advantages as skin substitutes because they can prevent fluid and proteins loss from the wound area, help in the removal of exudates, inhibit microbial infection, exhibit excellent anti–adhesion properties and guide endogenous cells to proliferate and remodel.
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Integrating novel technologies to fabricate smart scaffolds.
TL;DR: This review aims at summarizing conventional and novel scaffold fabrication techniques and the biomaterials used for tissue engineering and drug-delivery applications and suggests integrated technologies may lead to the generation of a new set of 3D scaffolds that satisfies all of the scaffolds' requirements for tissue-engineering applications and may contribute to their success in a long-term scenario.
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Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells
Chinnapaka Somaiah,Atul Kumar,Darilang Mawrie,Amit Sharma,Suraj Dasharath Patil,Jina Bhattacharyya,Rajaram Swaminathan,Bithiah Grace Jaganathan +7 more
TL;DR: Collagen will be a suitable matrix for large scale production of MSC with high survival rate and to obtain high osteogenic differentiation for therapy, according to the results of this study.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
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