Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Bio-inspired soft polystyrene nanotube substrate for rapid and highly efficient breast cancer-cell capture
TL;DR: Inspired by the fact that cells and their environments are composed of soft materials—something that has led to soft polymers being exploited in other biological applications, including tissue engineering—Wang and co-workers have replaced the stiff nanowires of their cell-capturing substrate with soft polystyrene nanotubes, leading to softness as an important factor in cell capture and detection.
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Tissue Engineering Approaches in the Design of Healthy and Pathological In Vitro Tissue Models.
TL;DR: 3D tissue-engineered models are expected to become useful tools in the preliminary testing and screening of drugs and therapies and in the investigation of the molecular mechanisms underpinning disease onset and progression.
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Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem cell differentiation.
Reeba S. Jacob,Dhiman Ghosh,Pradeep K. Singh,Santanu Kumar Basu,Narendra Nath Jha,Subhadeep Das,Pradip K. Sukul,Sachin P. Patil,Sadhana Sathaye,Ashutosh Kumar,Arindam Chowdhury,Sudip Malik,Shamik Sen,Samir K. Maji +13 more
TL;DR: The results indicate that small size, ease of custom synthesis, thixotropic nature makes these amyloid-based hydrogels ideally suited for biomaterial/nanotechnology applications.
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3D Spatiotemporal Mechanical Microenvironment: A Hydrogel-Based Platform for Guiding Stem Cell Fate.
TL;DR: The main mechanical cues that stem cells experience in their native microenvironment are summarized and recent advances in the design of hydrogel materials with spatiotemporally tunable mechanical properties for engineering 3D the spatiotsemporal mechanical microenvironment of stem cells are highlighted.
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Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering
Chetna Dhand,Seow Theng Ong,Neeraj Dwivedi,Silvia Marrero Diaz,Silvia Marrero Diaz,Jayarama Reddy Venugopal,Balchandar Navaneethan,Mobashar Hussain Urf Turabe Fazil,Shouping Liu,V. Seitz,Erich Wintermantel,Roger W. Beuerman,Seeram Ramakrishna,Seeram Ramakrishna,Navin Kumar Verma,Rajamani Lakshminarayanan +15 more
TL;DR: Among the two catecholamines, mats containing norepinephrine displayed superior mechanical, photoluminescence and biological properties than mats loaded with dopamine, which could potentially be utilized to repair and regenerate bone defects and injuries.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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