Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration
Giorgia Totonelli,Panagiotis Maghsoudlou,Massimo Garriboli,Johannes Riegler,Giuseppe Orlando,Alan J. Burns,Neil J. Sebire,Virpi V. Smith,Jonathan Fishman,Marco Ghionzoli,Mark Turmaine,Martin A. Birchall,Anthony Atala,Shay Soker,Mark F. Lythgoe,Alexander M. Seifalian,Agostino Pierro,Simon Eaton,Paolo De Coppi +18 more
TL;DR: Using a detergent-enzymatic treatment (DET), a new protocol is optimized in rats that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue, to strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.
Journal ArticleDOI
Engineering Stem Cell Self-organization to Build Better Organoids
TL;DR: How engineering efforts for guiding stem-cell-based development at multiple stages can form the basis for the assembly of highly complex and rationally designed self-organizing multicellular systems with increased robustness and physiological relevance is discussed.
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The control of stem cell morphology and differentiation by hydrogel surface wrinkles.
TL;DR: It is found that hMSCs attached to lamellar wrinkles spread by taking the shape of the pattern, exhibit high aspect ratios, and differentiate into an osteogenic lineage, in contrast, cells that attached inside the hexagonal patterns remain rounded with low spreading and differentiation into an adipogenic lineage.
Journal ArticleDOI
Matrix control of transforming growth factor-β function
TL;DR: In this paper, the authors discuss recent findings concerning the relationship of TGFβ, ECM molecules, and latent TGF-β activation and propose a model to resolve the 'TGFβ paradox.'
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Controlled Positioning of Cells in Biomaterials—Approaches Towards 3D Tissue Printing
TL;DR: In this review, current controlled material and cell positioning techniques are introduced highlighting approaches towards 3D tissue printing.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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