Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Stem cells and neurological diseases
TL;DR: A large body of evidence is emerging that cell therapy works by providing trophic or ‘chaperone’ support to the injured tissue and brain, and any cell therapy approach to neurological disease will have to be scalable and easily commercialized if it will have the necessary impact on public health.
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Nano-analytical electron microscopy reveals fundamental insights into human cardiovascular tissue calcification.
Sergio Bertazzo,Eileen Gentleman,Eileen Gentleman,Kristy L. Cloyd,Adrian H. Chester,Magdi H. Yacoub,Molly M. Stevens +6 more
TL;DR: Using nano-analytical electron microscopy techniques, valves, aortae and coronary arteries from patients with and without calcific cardiovascular disease are examined and spherical calcium phosphate particles are detected, regardless of the presence of calcific lesions, suggesting mineralized spherical particles may play a fundamental role in calcific lesion formation.
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Cyclic stretching of soft substrates induces spreading and growth
Yidan Cui,Feroz M. Hameed,Bo Yang,Kyunghee Lee,Catherine Qiurong Pan,Sungsu Park,Michael P. Sheetz +6 more
TL;DR: It is suggested that the repeated pulling from a soft matrix can substitute for a stiff matrix in stimulating spreading, stress fibre formation and growth.
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Cell mechanics: principles, practices, and prospects.
TL;DR: A generalized outline for measuring cell mechanical properties including loading protocols, tools, and data interpretation is described and it is explained how cell biomechanics research can be adopted by physicists, engineers, biologists, and clinicians alike.
Journal ArticleDOI
Hydrogels derived from central nervous system extracellular matrix.
Christopher J. Medberry,Peter M. Crapo,Bernard F. Siu,Christopher A. Carruthers,Matthew T. Wolf,Shailesh P. Nagarkar,Vineet Agrawal,Kristen E. Jones,Jeremy Kelly,Scott A. Johnson,Sachin Velankar,Simon C. Watkins,Michel Modo,Stephen F. Badylak +13 more
TL;DR: Results suggest that CNS-ECM hydrogels may provide supportive scaffolding to promote in vivo axonal repair, and may aid in tissue reconstruction efforts following complex neurologic trauma.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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