Matrix elasticity directs stem cell lineage specification.
Reads0
Chats0
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
More filters
Journal ArticleDOI
Mesenchymal stem cell-based therapy: a new paradigm in regenerative medicine.
Neeraj Kumar Satija,Vimal Kishor Singh,Yogesh Verma,Pallavi Gupta,Shilpa Sharma,Farhat Afrin,Menka Sharma,Pratibha Sharma,R. P. Tripathi,Gangenahalli U. Gurudutta +9 more
TL;DR: MSC transplantation, genetically modified MSC‐based therapy, tissue engineering using MSCs, and challenges and future prospects are reviewed.
Journal ArticleDOI
Electrically conductive gold nanoparticle-chitosan thermosensitive hydrogels for cardiac tissue engineering
Payam Baei,Sasan Jalili-Firoozinezhad,Sareh Rajabi-Zeleti,Mohammad Tafazzoli-Shadpour,Hossein Baharvand,Hossein Baharvand,Nasser Aghdami +6 more
TL;DR: It is demonstrated that incorporation of nanoscale electro-conductive GNPs into CS hydrogels enhances the properties of myocardial constructs and could find utilization for regeneration of other electroactive tissues.
Journal ArticleDOI
Mesenchymal Stem Cell Durotaxis Depends on Substrate Stiffness Gradient Strength
TL;DR: The data suggest that a functional actin cytoskeleton is required for migration whereas microtubules are required for directed migration, and imply that, in vivo, MSCs may preferentially accumulate in regions of high elastic modulus and make a greater contribution to tissue repairs in these locations.
Journal ArticleDOI
Cell-scaffold interactions in the bone tissue engineering triad.
TL;DR: The roles of progenitor cells, regulatory signals, and the biomaterials/scaffolds used to deliver them are discussed with a specific focus on the interaction between cell behaviour and scaffold structural properties.
Journal ArticleDOI
Versatile click alginate hydrogels crosslinked via tetrazine-norbornene chemistry.
Rajiv Desai,Sandeep T. Koshy,Scott A. Hilderbrand,David J. Mooney,David J. Mooney,Neel Joshi,Neel Joshi +6 more
TL;DR: In this article, click alginate hydrogels are used to encapsulate cells without damaging them using tetrazine and norbornene groups, which can be used in tissue engineering applications involving the stable encapsulation or delivery of cells or bioactive molecules.
References
More filters
Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
Related Papers (5)
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more