Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Defined three-dimensional microenvironments boost induction of pluripotency
Massimiliano Caiazzo,Yuya Okawa,Adrian Ranga,Alessandra Piersigilli,Yoji Tabata,Matthias P. Lutolf +5 more
TL;DR: It is found that the physical cell confinement imposed by the 3D microenvironment boosts reprogramming through an accelerated mesenchymal-to-epithelial transition and increased epigenetic remodelling and it is concluded that 3Dmicroenvironmental signals act synergistically with reprograming transcription factors to increase somatic plasticity.
Journal ArticleDOI
Bioinspired, calcium-free alginate hydrogels with tunable physical and mechanical properties and improved biocompatibility.
Changhyun Lee,Jisoo Shin,Jung Seung Lee,Eunkyoung Byun,Ji Hyun Ryu,Soong Ho Um,Dong Ik Kim,Haeshin Lee,Haeshin Lee,Seung Woo Cho +9 more
TL;DR: Alginate-catechol hydrogels enhanced the survival of various human primary cells including stem cells in the three-dimensional gel matrix, indicating that intrinsic cytotoxicity caused by divalent cations becomes negligible when employing catechol oxidation for alginate cross-linking.
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Concise Review: The Evolution of human pluripotent stem cell culture: From feeder cells to synthetic coatings†‡§
TL;DR: The establishment of defined culture conditions and synthetic matrices will facilitate studies to more precisely probe the molecular basis of pluripotent stem cell self‐renewal and differentiation and enable large‐scale expansion for future clinical applications.
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Dynamics of cell orientation
TL;DR: The model predicts many features observed in measurements of cellular forces and orientation including the increase with time of the cellular forces in the absence of applied stress and the consequent decrease of the force in the presence of quasi-static stresses.
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Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells
Yubing Sun,Koh Meng Aw Yong,Luis G. Villa-Diaz,Xiaoli Zhang,Weiqiang Chen,Renee Philson,Shinuo Weng,Haoxing Xu,Paul H. Krebsbach,Jianping Fu +9 more
TL;DR: It is shown that neural induction and caudalization of hPSCs can be accelerated by using a synthetic microengineered substrate system consisting of poly(dimethylsiloxane) micropost arrays (PMAs) with tunable mechanical rigidities.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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