Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Mechanotransduction at the cell-matrix interface.
TL;DR: This review discusses the complex mechanical signals of the ECM and outlines how IACs are involved in cellular sensing of these mechanical properties, focussing on the molecular mechanisms of key adhesion molecules.
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Carbon‐Based Nanomaterials for Tissue Engineering
TL;DR: Recent research advances in the effects of carbon nanomaterial‐based substrates on cellular behaviors, including cell adhesion, proliferation, and differentiation into osteo‐ or neural‐ lineages are summarized.
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Formin mDia1 senses and generates mechanical forces on actin filaments
TL;DR: Using the viscous drag of a microfluidic flow, calibrated piconewton pulling forces are applied to individual actin filaments that are being elongated at their barbed end by surface-anchored mDia1 proteins to suggest that formin function in cells is tightly coupled to the mechanical activity of other machineries.
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Theoretical and computational hierarchical nanomechanics of protein materials: Deformation and fracture
TL;DR: Progress in understanding the deformation and fracture mechanisms of hierarchical protein materials is reviewed by using a materials science approach to develop structure-process-property relations, an effort defined as materiomics.
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Matrix Strains Induced by Cells: Computing How Far Cells Can Feel
TL;DR: Three-dimensional cell morphologies that model stem cell-derived neurons, myoblasts, and osteoblasts show that a cylinder-shaped myoblast induces the highest strains, consistent with the prominent contractility of muscle.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
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Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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