Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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The design of reversible hydrogels to capture extracellular matrix dynamics
TL;DR: The extracellular matrix (ECM) is a dynamic environment that constantly provides physical and chemical cues to embedded cells, and emerging trends include the use of molecular photoswitches or biomolecule hybridization to control polymer chain conformation.
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Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography.
Robert Gauvin,Ying-Chieh Chen,Ying-Chieh Chen,Ying-Chieh Chen,Jin Woo Lee,Pranav Soman,Pinar Zorlutuna,Pinar Zorlutuna,Jason W. Nichol,Jason W. Nichol,Hojae Bae,Hojae Bae,Shaochen Chen,Ali Khademhosseini,Ali Khademhosseini,Ali Khademhosseini +15 more
TL;DR: Results demonstrated that the interconnectivity of pores allowed for uniform human umbilical vein endothelial cells (HUVECs) distribution and proliferation in the scaffolds, leading to high cell density and confluency at the end of the culture period.
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Functionalization, preparation and use of cell-laden gelatin methacryloyl–based hydrogels as modular tissue culture platforms
Daniela Loessner,Christoph Meinert,Elke Kaemmerer,Laure Martine,Kan Yue,Kan Yue,Peter A. Levett,Travis J. Klein,Ferry P.W. Melchels,Ferry P.W. Melchels,Ferry P.W. Melchels,Ali Khademhosseini,Dietmar W. Hutmacher +12 more
TL;DR: The gelatin methacryloyl (GelMA)-based hydrogels as discussed by the authors combine the biocompatibility of natural matrices with the reproducibility, stability and modularity of synthetic biomaterials.
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Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation
Ana Maria Pasapera,Ian C. Schneider,Ian C. Schneider,Erin Rericha,Erin Rericha,David D. Schlaepfer,Clare M. Waterman,Clare M. Waterman +7 more
TL;DR: FAK-mediated myosin-dependent paxillin phosphorylation is necessary to bring vinculin to maturing focal adhesions, reinforcing the link between the cytoskeleton and the ECM.
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Adaptable Hydrogel Networks with Reversible Linkages for Tissue Engineering
Huiyuan Wang,Sarah C. Heilshorn +1 more
TL;DR: An overview of adaptable‐hydrogel design considerations and linkage selections is presented, with a focus on various cell‐compatible crosslinking mechanisms that can be exploited to form adaptable hydrogels for tissue engineering.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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