Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Bio-inks for 3D bioprinting: recent advances and future prospects
Ilze Donderwinkel,Ilze Donderwinkel,Jcm Jan van Hest,Jcm Jan van Hest,Neil R. Cameron,Neil R. Cameron +5 more
TL;DR: All the currently used bio-printing inks, including polymeric hydrogels, polymer bead microcarriers, cell aggregates and extracellular matrix proteins, and photocrosslinkable and thermoresponsive materials are described.
Journal ArticleDOI
A new micropatterning method of soft substrates reveals that different tumorigenic signals can promote or reduce cell contraction levels.
Qingzong Tseng,Irène Wang,Eve Duchemin-Pelletier,Ammar Azioune,Nicolas Carpi,Jie Gao,Odile Filhol,Matthieu Piel,Manuel Théry,Martial Balland +9 more
TL;DR: This work developed a new method to micropattern extracellular matrix proteins on poly-acrylamide gels in order to simultaneously control cell geometry and mechanics and found that, contrary to the current view, all transformation phenotypes were not always associated to an increased level of cell contractility.
Journal ArticleDOI
Mechanical forces in the immune system
TL;DR: The current understanding of how mechanical force regulates cell-surface receptor activation, cell migration, intracellular signalling and intercellular communication is reviewed, highlighting the biological ramifications of these effects in various immune cell types.
Journal ArticleDOI
Molecular mechanisms of cellular mechanosensing
TL;DR: The observations show that the accumulation kinetics of each protein may be explained by its molecular mechanisms, and that protein accumulation and the cell's viscoelastic state can explain cell contraction against mechanical load.
Matrix elasticity of void-forming hydrogels controls transplanted-stem-cell-mediated bone formation
Evi Lippens,Kangwon Lee,Manav Mehta,Sandeep T. Koshy,Max Darnell,Rajiv Desai,Christopher M. Madl,Maria M. Xu,Xuanhe Zhao,Ovijit Chaudhuri,Catia S. Verbeke,Woo Seob Kim,Karen Alim,Akiko Mammoto,Donald E. Ingber,Georg N. Duda,David J. Mooney,Nathaniel Huebsch +17 more
TL;DR: By developing injectable, void-forming hydrogels that decouple pore formation from elasticity, this work shows that mesenchymal stem cell osteogenesis in vitro, and cell deployment in vitro and in vivo, can be controlled by modifying the hydrogel's elastic modulus or its chemistry.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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