Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Neurons sense nanoscale roughness with nanometer sensitivity
Virgilio Brunetti,Gabriele Maiorano,Loris Rizzello,Barbara Sorce,Stefania Sabella,Roberto Cingolani,Pier Paolo Pompa +6 more
TL;DR: It is demonstrated that neurons sense and actively respond to the surface nanotopography, with a surprising sensitivity to variations of few nanometers, delivering a simple and elegant approach for the design and development of biomaterials with precise nanostructure-triggered biological responses.
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3D Bioprinting: from Benches to Translational Applications.
Marcel A. Heinrich,Marcel A. Heinrich,Wanjun Liu,Wanjun Liu,Andrea Jimenez,Jingzhou Yang,Ali Akpek,Ali Akpek,Ali Akpek,Xiao Liu,Xiao Liu,Qingmeng Pi,Qingmeng Pi,Xuan Mu,Ning Hu,Ning Hu,Raymond M. Schiffelers,Jai Prakash,Jingwei Xie,Yu Shrike Zhang +19 more
TL;DR: The history of bioprinting and the most recent advances in instrumentation and methods are covered, and the requirements for bioinks and cells to achieve optimal fabrication of biomimetic constructs are focused on.
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Mimicking Stem Cell Niches to Increase Stem Cell Expansion
TL;DR: Stem cell niches regulate lineage-specific stem cell self-renewal versus differentiation in vivo and are composed of supportive cells and extracellular matrix components arranged in a three-dimensional topography of controlled stiffness in the presence of oxygen and growth factors.
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Microfluidic platforms for mechanobiology
TL;DR: In this paper, the authors discuss how microfluidics has transformed the study of mechanotransduction and discuss new biological insights that have been elucidated by using micro-fluidic experiments.
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Single-cell response to stiffness exhibits muscle-like behavior.
Démosthène Mitrossilis,Jonathan Fouchard,Axel Guiroy,Nicolas Desprat,Nicolas Rodriguez,Ben Fabry,Atef Asnacios +6 more
TL;DR: An unexpected mechanism of rigidity sensing is revealed, whereby the contractile acto-myosin units themselves can act as sensors, and could thus coordinate local activity of adhesion complexes and guide cell migration along rigidity gradients.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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