Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Finding the weakest link: exploring integrin-mediated mechanical molecular pathways.
TL;DR: This work reconstructs the mechanical pathway followed by these forces from matrix proteins to actin through integrins and adaptor proteins, and examines which of the proteins in the network can participate in mechanotransduction and which are simply responsible for transmitting forces in a dynamic network.
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In vivo quantification of spatially varying mechanical properties in developing tissues
Friedhelm Serwane,Alessandro Mongera,Payam Rowghanian,David A. Kealhofer,Adam A. Lucio,Zachary Hockenbery,Zachary Hockenbery,Otger Campàs +7 more
TL;DR: Using a technique that employs biocompatible, magnetically responsive ferrofluid microdroplets as local mechanical actuators and allows quantitative spatiotemporal measurements of mechanical properties in vivo, it is shown that vertebrate body elongation entails spatially varying tissue mechanics along the anteroposterior axis.
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Directing stem cell fate on hydrogel substrates by controlling cell geometry, matrix mechanics and adhesion ligand composition.
TL;DR: A microengineering strategy to vary single cell geometry and the composition of adhesion ligands - on substrates that approximate the mechanical properties of soft tissues - to study adipogenesis and neurogenesis in adherent mesenchymal stem cells is demonstrated.
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Strength Dependence of Cadherin-Mediated Adhesions
Benoit Ladoux,Benoit Ladoux,Ester Anon,Mireille Lambert,Mireille Lambert,Aleksandr Rabodzey,Pascal Hersen,Axel Buguin,Axel Buguin,Pascal Silberzan,René-Marc Mège,René-Marc Mège +11 more
TL;DR: A quantitative analysis of the cells adhering on the cadherin-coated surfaces shows that forces are correlated with the formation of Cadherin adhesions, and these findings are consistent with a mechanosensitive regulation of cadher in-mediated intercellular junctions through the cellular contractile machinery.
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Injectable biodegradable hydrogels: progress and challenges
TL;DR: An overview of state-of-the-art strategies towards constructing a rational design of injectable biodegradable hydrogels for protein drug delivery and tissue engineering is provided.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
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Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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