Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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Multicomponent peptide assemblies
TL;DR: The design, structure, emergent properties, and applications for these multicomponent assemblies are presented in order to illustrate the potential of these formulations as sophisticated next-generation bio-inspired materials.
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Endothelial actin and cell stiffness is modulated by substrate stiffness in 2D and 3D.
TL;DR: The data demonstrate that extracellular substrate stiffness regulates endothelial stiffness in both three- and two-dimensional environments, though the range of stiffnesses that cells respond to vary significantly in different environments.
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Self-assembled amino acids and dipeptides as noncovalent hydrogels for tissue engineering
Derek M. Ryan,Bradley L. Nilsson +1 more
TL;DR: This review will critically assess current progress in the use of minimal self-assembling peptides and functionalized amino acids to create hydrogels, with a focus on the challenges of understanding the structure and function of these materials and on the outlook for theUse of these modular and dynamic materials as robust networks for tissue engineering.
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Microfluidic-enhanced 3D bioprinting of aligned myoblast-laden hydrogels leads to functionally organized myofibers in vitro and in vivo
Marco Costantini,Stefano Testa,Pamela Mozetic,Andrea Barbetta,Claudia Fuoco,Ersilia Fornetti,Francesco Tamiro,Sergio Bernardini,Jakub Jaroszewicz,Wojciech Święszkowski,Marcella Trombetta,Luisa Castagnoli,Dror Seliktar,Piotr Garstecki,Gianni Cesareni,Stefano Cannata,Alberto Rainer,Cesare Gargioli +17 more
TL;DR: The results demonstrate an enhanced myogenic differentiation with the formation of parallel aligned long-range myotubes within the 3D bioprinted fibers, further revealing maturation, sarcomerogenesis, and functionality.
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Nanoparticles and innate immunity: new perspectives on host defence
Diana Boraschi,Paola Italiani,Roberto Palomba,Paolo Decuzzi,Albert Duschl,Bengt Fadeel,S. Moein Moghimi +6 more
TL;DR: N nanoparticle interactions with different cell types and soluble mediators belonging to the innate immune system are discussed, where a better understanding of these processes could pave the way for improved vaccination strategies.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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