Matrix elasticity directs stem cell lineage specification.
TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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The Hippo pathway effectors TAZ and YAP in development, homeostasis and disease
TL;DR: An overview of regulatory mechanisms and important developmental processes controlled by TAZ and YAP is provided and it is outlined that TAZ/YAP activity is regulated by mechanical and cytoskeletal cues as well as by various extracellular factors.
Journal ArticleDOI
Design, fabrication and characterization of PCL electrospun scaffolds—a review
Amaia Cipitria,Amaia Cipitria,Anthony D. Skelton,Tim R. Dargaville,Paul D. Dalton,Paul D. Dalton,Dietmar W. Hutmacher,Dietmar W. Hutmacher +7 more
TL;DR: In this paper, a review summarizes literature on poly(3-caprolactone) and selected blends, and provides extensive descriptions of the broad range of parameters used in manufacturing such electrospun fibers.
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Bone marrow derived mesenchymal stem cells facilitate engineering of long-lasting functional vasculature
TL;DR: Human bone marrow-derived mesenchymal stem cells (hMSCs) are perivascular cell precursors and may serve as an attractive source of cells for use in vascular tissue engineering and for the study of periv vascular cell differentiation.
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Rejuvenation of the muscle stem cell population restores strength to injured aged muscles
Benjamin D. Cosgrove,Penney M. Gilbert,Ermelinda Porpiglia,Foteini Mourkioti,Steven P. Lee,Stéphane Y. Corbel,Michael E. Llewellyn,Scott L. Delp,Helen M. Blau +8 more
TL;DR: Subjecting theMuSC population from aged mice to transient inhibition of p38α and p38β in conjunction with culture on soft hydrogel substrates rapidly expands the residual functional MuSC population, rejuvenating its potential for regeneration and serial transplantation as well as strengthening of damaged muscles of aged mice.
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Directing osteogenic and myogenic differentiation of MSCs: interplay of stiffness and adhesive ligand presentation
TL;DR: The modulation of myogenic and osteogenic transcription factors by various ECM proteins demonstrates that substrate stiffness alone does not direct stem cell lineage specification, which has important implications in the development of tailored biomaterial systems that more closely mimic the microenvironment found in native tissues.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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