Journal ArticleDOI
Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease.
Barbara W Sódar,Árpád Kovács,Tamás Visnovitz,Éva Pállinger,Károly Vékey,Gabriella Pocsfalvi,Lilla Turiák,Edit I. Buzás +7 more
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TLDR
R rigor in data reproducibility, critical data analysis, awareness of potential pitfalls, standardization and benchmarking are required for extracellular vesicle research to fulfil the current expectation that these subcellular structures can become a valid source of next generation biomarkers.Abstract:
Introduction: Extracellular vesicles are emerging sources of biomarkers for modern preventive and precision medicine. Extracellular vesicles in body fluids offer a unique opportunity for integrative biomarker approaches due to their complex biocargo that includes proteins, lipids, nucleic acids and metabolites. Mass spectrometry-based proteomics data suggest that a significant portion of human proteins are sorted into extracellular vesicles and amenable for biomarker discovery schemes.Areas covered: this review focuses on key aspects of isolation, quality control and subsequent analysis of blood plasma- and conditioned medium-derived extracellular vesicle proteins, and summarizes the current state-of-the-art in the field. Furthermore, it provides introduction and guidelines for mass spectrometry-based proteomic analysis of extracellular vesicles.Expert commentary: Comparison of newly developed isolation and purification techniques with classical ultracentrifugation-based approaches are highly reco...read more
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Journal ArticleDOI
Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches
TL;DR: An overview of available technologies for EV analysis is provided by describing their working principles, assessing their utility in EV research and summarising their potential and limitations.
Journal ArticleDOI
Extracellular Vesicle as a Source of Alzheimer's Biomarkers: Opportunities and Challenges.
TL;DR: In this article, the role of extracellular vesicles (EVs) in Alzheimer's disease (AD) is discussed and the development of valid EV-associated biomarkers is discussed.
Journal ArticleDOI
Extracellular Vesicles and Their Potential Use in Monitoring Cancer Progression and Therapy: The Contribution of Proteomics
Maria Concetta Cufaro,Damiana Pieragostino,Paola Lanuti,Claudia Rossi,Ilaria Cicalini,Luca Federici,Vincenzo De Laurenzi,Piero Del Boccio +7 more
TL;DR: This review presents the state-of-the-art of EVs isolation, purification, and characterization for omics studies, with a particular focus to their potential use in monitoring cancer progression and therapy.
Journal ArticleDOI
Extracellular vesicles as cancer liquid biopsies: from discovery, validation, to clinical application
TL;DR: The potential application of EV biomarkers for diagnosis of major cancer types is focused on, discussing approaches for EV biomarker discovery and verification, EV clinical assay development, analytical and clinical validation, clinical trials, regulatory submission, and end user utilization for the intended clinical application.
Journal ArticleDOI
Exosome Processing and Characterization Approaches for Research and Technology Development
James J. Lai,Zoe L Chau,Sheng-You Chen,John J. Hill,Katalin V. Korpany,Nai-Wen Liang,Li-Han Lin,Yi-Hsuan Lin,Joanne K Liu,Yu-Chung Liu,Ruby Lunde,Wei-Ting Shen +11 more
TL;DR: A detailed introduction to exosomes, including their physical and chemical properties, roles in normal biological processes and in disease progression, and summarizes some of the on‐going clinical trials are provided.
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