Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection
Rafael D. Mesquita,Raquel J. Vionette-Amaral,Carl Lowenberger,Rolando Rivera-Pomar,Fernando A. Monteiro,Fernando A. Monteiro,Patrick Minx,John Spieth,A. Bernardo Carvalho,Francisco Panzera,Daniel Lawson,André Q. Torres,André Q. Torres,José M. C. Ribeiro,Marcos Henrique Ferreira Sorgine,Robert M. Waterhouse,Michael J. Montague,Fernando Abad-Franch,Michele Alves-Bezerra,Laurence Rodrigues do Amaral,Helena Araujo,Ricardo Nascimento Araújo,Ricardo Nascimento Araújo,L. Aravind,Georgia C. Atella,Patrícia Azambuja,Patrícia Azambuja,Mateus Berni,Paula R. Bittencourt-Cunha,Glória R.C. Braz,Gustavo M. Calderón-Fernández,Claudia M. A. Carareto,Mikkel B. Christensen,Igor Rodrigues da Costa,Samara G. da Costa,Marilvia Dansa,Carlos R. O. Daumas-Filho,Iron F. De-Paula,Felipe A. Dias,George Dimopoulos,Scott J. Emrich,Natalia Esponda-Behrens,Patrícia Fampa,Rita D. Fernandez-Medina,Rodrigo Nunes da Fonseca,Marcio Fontenele,Catrina Fronick,Lucinda Fulton,Ana Caroline P. Gandara,Eloi S. Garcia,Eloi S. Garcia,Fernando A. Genta,Fernando A. Genta,Gloria I. Giraldo-Calderón,Bruno Gomes,Bruno Gomes,Katia C. Gondim,Adriana Granzotto,Alessandra A. Guarneri,Alessandra A. Guarneri,Roderic Guigó,Myriam Harry,Daniel S.T. Hughes,Willy Jablonka,Emmanuelle Jacquin-Joly,M. Patricia Juárez,Leonardo Koerich,Angela B. Lange,Jose Manuel Latorre-Estivalis,Jose Manuel Latorre-Estivalis,Andrés Lavore,Gena G. Lawrence,Gena G. Lawrence,Cristiano Lazoski,Claudio R. Lazzari,Raphael R.S. Lopes,Marcelo G. Lorenzo,Marcelo G. Lorenzo,Magda D. Lugon,David Majerowicz,Paula L. Marcet,Marco Mariotti,Hatisaburo Masuda,Karyn Megy,Ana C.A. Melo,Fanis Missirlis,Theo Mota,Fernando G. Noriega,Marcela Nouzova,Rodrigo Dutra Nunes,Raquel L.L. Oliveira,Gilbert Oliveira-Silveira,Sheila Ons,Ian Orchard,Lucia Pagola,Gabriela O. Paiva-Silva,Agustina Pascual,Márcio G. Pavan,Nicolás Pedrini,Alexandre A. Peixoto,Alexandre A. Peixoto,Marcos H. Pereira,Marcos H. Pereira,Andrew Pike,Carla Polycarpo,Francisco Prosdocimi,Rodrigo Ribeiro-Rodrigues,Hugh M. Robertson,Ana Paula Salerno,Didier Salmon,Didac Santesmasses,Renata Schama,Renata Schama,Eloy S. Seabra-Junior,Lívia Silva-Cardoso,Mário A.C. Silva-Neto,Matheus Souza-Gomes,Marcos Sterkel,Mabel L. Taracena,Marta Tojo,Zhijian Jake Tu,Jose M. C. Tubio,Raul Ursic-Bedoya,Thiago M. Venancio,Thiago M. Venancio,Ana Beatriz Walter-Nuno,Derek Wilson,Wesley C. Warren,Richard K. Wilson,Erwin Huebner,Ellen M. Dotson,Pedro L. Oliveira +131 more
TLDR
The first genome sequence of a nondipteran insect vector of an important human parasitic disease is described, which provides critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.Abstract:
Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (∼ 702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.read more
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Journal ArticleDOI
Insect immunology and hematopoiesis.
TL;DR: How the immune and circulatory systems have co-adapted to combat infection, how hemocyte replication and differentiation takes place (hematopoiesis), how an infection prepares an insect for a subsequent infection (immune priming), how environmental factors such as temperature and the age of the insect impact the immune response, and how social immunity protects entire groups are discussed.
Journal ArticleDOI
The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance
Wenbo Chen,Daniel K. Hasegawa,Daniel K. Hasegawa,Navneet Kaur,Adi Kliot,Patricia V. Pinheiro,Patricia V. Pinheiro,Patricia V. Pinheiro,Jun-Bo Luan,Marcus C. Stensmyr,Yi Zheng,Wenli Liu,Honghe Sun,Yimin Xu,Yuan Luo,Angela Kruse,Xiaowei Yang,Svetlana Kontsedalov,Galina Lebedev,Tonja W. Fisher,David R. Nelson,Wayne B. Hunter,Judith K. Brown,Georg Jander,Michelle Cilia,Michelle Cilia,Michelle Cilia,Angela E. Douglas,Murad Ghanim,Alvin M. Simmons,William M. Wintermantel,Kai Shu Ling,Zhangjun Fei,Zhangjun Fei +33 more
TL;DR: The B. tabaci genome contains numerous genetic novelties, including expansions in gene families associated with insecticide resistance, detoxification and virus transmission, as well as numerous horizontally transferred genes from bacteria and fungi.
Journal ArticleDOI
Phylogenomics and the evolution of hemipteroid insects.
Kevin P. Johnson,Christopher H. Dietrich,Frank Friedrich,Rolf G. Beutel,Benjamin Wipfler,Ralph S. Peters,Julie M. Allen,Julie M. Allen,Malte Petersen,Alexander Donath,Kimberly K. O. Walden,Alexey M. Kozlov,Lars Podsiadlowski,Christoph Mayer,Karen Meusemann,Karen Meusemann,Alexandros Vasilikopoulos,Robert M. Waterhouse,Stephen L. Cameron,Christiane Weirauch,Daniel R. Swanson,Diana M. Percy,Diana M. Percy,Nate B. Hardy,Irene Terry,Shanlin Liu,Xin Zhou,Bernhard Misof,Hugh M. Robertson,Kazunori Yoshizawa +29 more
TL;DR: The results indicated that thrips (Thysanoptera) are the closest living relatives of true bugs and allies (Hemiptera) and that hemipteroid insects started diversifying before the Carboniferous period, over 365 million years ago.
Journal ArticleDOI
A look inside odorant-binding proteins in insect chemoreception.
TL;DR: The role of OBPs in insect chemoreception is discussed, as well as their promising application as molecular targets for the development of new strategies for insect population management and other technological purposes.
Journal ArticleDOI
Hemimetabolous genomes reveal molecular basis of termite eusociality
Mark C Harrison,Evelien Jongepier,Hugh M. Robertson,Nicolas Arning,Tristan Bitard-Feildel,Hsu Chao,Christopher P. Childers,Huyen Dinh,Harshavardhan Doddapaneni,Shannon Dugan,Johannes Gowin,Johannes Gowin,Carolin Greiner,Carolin Greiner,Yi Han,Haofu Hu,Daniel S.T. Hughes,Ann Kathrin Huylmans,Carsten Kemena,Lukas P.M. Kremer,Sandra L. Lee,Alberto Lopez-Ezquerra,Ludovic Mallet,Jose M. Monroy-Kuhn,Annabell Moser,Shwetha C. Murali,Donna M. Muzny,Saria Otani,Maria-Dolors Piulachs,Monica F. Poelchau,Jiaxin Qu,Florentine Schaub,Ayako Wada-Katsumata,Kim C. Worley,Qiaolin Xie,Guillem Ylla,Michael Poulsen,Richard A. Gibbs,Coby Schal,Stephen Richards,Xavier Bellés,Judith Korb,Judith Korb,Erich Bornberg-Bauer +43 more
TL;DR: Dramatic adaptive changes in genes underlying the production and perception of pheromones confirm the importance of chemical communication in the termites and provide insights into the evolutionary signatures of termite eusociality.
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