Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles.
André Cronemberger Andrade,Martin Wolf,Heide-Marie Binder,Fausto Gueths Gomes,Felix Manstein,Patricia Ebner-Peking,Rodolphe Poupardin,Robert Zweigerdt,Katharina Schallmoser,Dirk Strunk +9 more
TLDR
In this paper, the authors investigated the effect of hypoxia on extracellular vesicles (EVs) in human induced pluripotent stem cells (iPSC) culture.Abstract:
Stem cells secrete paracrine factors including extracellular vesicles (EVs) which can mediate cellular communication and support the regeneration of injured tissues. Reduced oxygen (hypoxia) as a key regulator in development and regeneration may influence cellular communication via EVs. We asked whether hypoxic conditioning during human induced pluripotent stem cell (iPSC) culture effects their EV quantity, quality or EV-based angiogenic potential. We produced iPSC-EVs from large-scale culture-conditioned media at 1%, 5% and 18% air oxygen using tangential flow filtration (TFF), with or without subsequent concentration by ultracentrifugation (TUCF). EVs were quantified by tunable resistive pulse sensing (TRPS), characterized according to MISEV2018 guidelines, and analyzed for angiogenic potential. We observed superior EV recovery by TFF compared to TUCF. We confirmed hypoxia efficacy by HIF-1α stabilization and pimonidazole hypoxyprobe. EV quantity did not differ significantly at different oxygen conditions. Significantly elevated angiogenic potential was observed for iPSC-EVs derived from 1% oxygen culture by TFF or TUCF as compared to EVs obtained at higher oxygen or the corresponding EV-depleted soluble factor fractions. Data thus demonstrate that cell-culture oxygen conditions and mode of EV preparation affect iPSC-EV function. We conclude that selecting appropriate protocols will further improve production of particularly potent iPSC-EV-based therapeutics.read more
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Adherence to minimal experimental requirements for defining extracellular vesicles and their functions.
TL;DR: This paper performed a systematic review using a text mining approach to assess adherence to the minimal information for studies of extracellular vesicles (MISEV) guidelines for assuring and improving EV research quality.
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Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles
Kekoolani S Visan,Richard J. Lobb,Sunyoung Ham,Luize G. Lima,Carlos Palma,Chai Pei Zhi Edna,Li-ying Wu,Harsha T Gowda,Keshava K. Datta,Gunter Hartel,Carlos Salomon,Andreas Möller +11 more
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Synergy of Human Platelet-Derived Extracellular Vesicles with Secretome Proteins Promotes Regenerative Functions
Fausto Gueths Gomes,André Cronemberger Andrade,Martin Wolf,Sarah Hochmann,Linda M. Krisch,Nicole Maeding,Christof Regl,Rodolphe Poupardin,Patricia Ebner-Peking,Christian G. Huber,Nicole Meisner-Kober,Katharina Schallmoser,Dirk Strunk +12 more
TL;DR: It is concluded that permissive isolation technology, such as TFF, and better understanding of the mechanism of EV corona function are required to realize the complete potential of platelet-based regenerative therapies.
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Stem Cell-Derived Extracellular Vesicles as Potential Therapeutic Approach for Acute Kidney Injury
Marco Quaglia,Guido Merlotti,A. Colombatto,Stefania Bruno,Alessandra Stasi,Rossana Franzin,Giuseppe Castellano,Elena Grossini,Vito Fanelli,Vincenzo Cantaluppi +9 more
TL;DR: The state-of-the-art knowledge of therapeutic approaches with stem cell-derived extracellular vesicles for different forms of acute kidney injury (toxic, ischemic or septic) dissecting their cytoprotective, regenerative and immunomodulatory properties are analyzed.
Journal ArticleDOI
Understanding the extracellular vesicle surface for clinical molecular biology
TL;DR: The current understanding of the mechanical, electrostatic and molecular properties of the EV surface that offer significant biomarker potential and contribute to a highly dynamic interactome are described.
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TL;DR: The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities, and a checklist is provided with summaries of key points.
Journal ArticleDOI
Shedding light on the cell biology of extracellular vesicles.
TL;DR: Extracellular vesicles are now considered as an additional mechanism for intercellular communication, allowing cells to exchange proteins, lipids and genetic material.
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The biology, function, and biomedical applications of exosomes
TL;DR: The intrinsic properties of exosomes in regulating complex intracellular pathways has advanced their potential utility in the therapeutic control of many diseases, including neurodegenerative conditions and cancer.
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Oxygen in Stem Cell Biology: A Critical Component of the Stem Cell Niche
TL;DR: This work has identified a broader spectrum of stem cells influenced by hypoxia that includes cancer stem cells and induced pluripotent stem cells, and elucidate an added dimension of stem cell control within the niche.