Journal ArticleDOI
Transferrin recycling in reticulocytes: pH and iron are important determinants of ligand binding and processing
TLDR
Receptor-mediated movement of Tf through acid intracellular compartments provides a mechanism to remove iron from Tf and for apoTf to remain receptor-bound for externalization to the cell surface and subsequent dissociation.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1983-06-15. It has received 190 citations till now. The article focuses on the topics: Transferrin.read more
Citations
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Journal ArticleDOI
Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes
TL;DR: The data suggest that transferrin is internalized via coated pits and vesicles and demonstrate thatTransferrin and its receptor are recycled back to the plasma membrane after endocytosis, and are shown to be lysosomal in nature.
Journal ArticleDOI
The molecular mechanisms of the metabolism and transport of iron in normal and neoplastic cells.
Des R. Richardson,Prem Ponka +1 more
TL;DR: Iron uptake by mammalian cells is mediated by the binding of serum Tf to the TfR, and recent work has suggested that the short-lived messenger molecule, NO, can affect cellular Fe metabolism via its interaction with IRP1.
Book ChapterDOI
A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods
TL;DR: This chapter reveals the protocol and key insights into the isolation, purification and characterization of exosomes, distinct from shed microvesicles and apoptotic blebs, and concludes that density-based separation (DG-Exos) provides significant advantages for exosome isolation when the use of immunoaffinity capture is limited.
Journal ArticleDOI
Exosomes: Looking back three decades and into the future
TL;DR: The initial experiments that led to the discovery of exosomes are discussed and some of the exciting current directions in the field are highlighted.
Journal ArticleDOI
Biological Functions and Current Advances in Isolation and Detection Strategies for Exosome Nanovesicles.
Kseniia Boriachek,Nazmul Islam,Andreas Möller,Carlos Salomon,Carlos Salomon,Nam-Trung Nguyen,Shahriar A. Hossain,Shahriar A. Hossain,Yusuke Yamauchi,Yusuke Yamauchi,Muhammad J. A. Shiddiky +10 more
TL;DR: The major biological functions, significance, and potential role of exosomes as biomarkers and therapeutics are discussed and an overview of the most commonly used techniques for exosome analysis, highlighting the major technical challenges and limitations of existing techniques, is presented.
References
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Journal ArticleDOI
Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling.
TL;DR: This model explains how chloroquine, which raises intralysosomal pH, can disrupt both the intracellular pathway for newly synthesized acid hydrolases, and the one for uptake of exogenous enzyme by cell surface pinocytosis receptors.
Journal ArticleDOI
Rapid acidification of endocytic vesicles containing α2-macroglobulin
TL;DR: Results indicate that endocytic vesicles become acidic prior to fusion with lysosomes, as measured by the pH changes in the microenvironment of internalized ligands following receptor-mediated endocytosis.
Journal ArticleDOI
Receptor-mediated pinocytosis of mannose glycoconjugates by macrophages: Characterization and evidence for receptor recycling
TL;DR: The results, taken together with the observation that cycloheximide has no effect on ligand uptake, suggest that receptors must be spared from degradation and that reutilization of receptors probably occurs.
Journal ArticleDOI
Weak bases and ionophores rapidly and reversibly raise the pH of endocytic vesicles in cultured mouse fibroblasts.
TL;DR: It is shown that endocytic vesicles become acidic at very early times (i.e., within 5-7 min of continuous uptake at 37 degrees C) and could be reversed by removal of the perturbant.
Journal ArticleDOI
Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells.
TL;DR: It is now possible to design selection strategies for the isolation of mutant teratocarcinoma stem cells carrying different genetic lesions affecting the transferrin-mediated pathway as described here, and to enable construction of mice carrying specific iron transport defects.
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