University of Hawaii at Manoa

About: University of Hawaii at Manoa is a education organization based out in Honolulu, Hawaii, United States. It is known for research contribution in the topics: Population & Poison control. The organization has 13693 authors who have published 25161 publications receiving 1023924 citations.

Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa

Abstract: Chromosome stability was maintained in mouse spermatozoa after freeze-drying or freezing without cryoprotection in a simple Tris⋅HCl buffer containing EGTA (50 mM) and NaCl (50 mM). The ability of spermatozoa to activate oocytes spontaneously was not destroyed by freeze-drying or freezing without cryoprotection in this solution. Embryos derived after injecting oocytes with sperm heads from rehydrated freeze-dried and from thawed spermatozoa developed normally. Provided the DNA integrity of the sperm nucleus is maintained, embryos can be generated by the intracytoplasmic sperm injection technique (ICSI) from severely damaged spermatozoa that are no longer capable of normal physiological activity. This procedure was effective for preserving spermatozoa from strains (C57BL/6J, 129/SvJ, and BALB/c) in which the fertility of spermatozoa frozen conventionally is extremely poor. The technique provides an effective means of storing mouse spermatozoa from many different inbred, mutant, and transgenic strains for biomedical research.

Multi-stage onset of the summer monsoon over the western North Pacific

Abstract: The climatological summer monsoon onset displays a distinct step wise northeastward movement over the South China Sea and the western North Pacific (WNP) (110°–160°E, 10°–20°N). Monsoon rain commences over the South China Sea-Philippines region in mid-May, extends abruptly to the southwestern Philippine Sea in early to mid-June, and finally penetrates to the northeastern part of the domain around mid-July. In association, three abrupt changes are identified in the atmospheric circulation. Specifically, the WNP subtropical high displays a sudden eastward retreat or quick northward displacement and the monsoon trough pushes abruptly eastward or northeastward at the onset of the three stages. The step wise movement of the onset results from the slow northeastward seasonal evolution of large-scale circulation and the phase-locked intraseasonal oscillation (ISO). The seasonal evolution establishes a large-scale background for the development of convection and the ISO triggers deep convection. The ISO over the WNP has a dominant period of about 20–30 days. This determines up the time interval between the consecutive stages of the monsoon onset. From the atmospheric perspective, the seasonal sea surface temperature (SST) change in the WNP plays a critical role in the northeastward advance of the onset. The seasonal northeastward march of the warmest SST tongue (SST exceeding 29.5 °C) favors the northeastward movement of the monsoon trough and the high convective instability region. The seasonal SST change, in turn, is affected by the monsoon through cloud-radiation and wind-evaporation feedbacks.

Icelandic analogs to Martian flood lavas

Abstract: We report on new field observations from Icelandic lava flows that have the same surface morphology as many Martian flood lava flows. The Martian flood lavas are characterized by a platy-ridged surface morphology whose formation is not well understood. The examples on Mars include some of the most pristine lava on the planet and flows >1500 km long. The surfaces of the flows are characterized by (1) ridges tens of meters tall and wide and hundreds of meters long, (2) plates hundreds of meters to kilometers across that are bounded by ridges, (3) smooth surfaces broken into polygons several meters across and bowed up slightly in the center, (4) parallel grooves 1–10 km long cut into the flow surface by flow past obstacles, and (5) inflated pahoehoe margins. The Icelandic examples we examined (the 1783–1784 Laki Flow Field, the Burfells Lava Flow Field by Lake Myvatn, and a lava flow from Krafla Volcano) have all these surface characteristics. When examined in detail, we find that the surfaces of the Icelandic examples are composed primarily of disrupted pahoehoe. In some cases the breccia consists of simple slabs of pahoehoe lava; in other cases it is a thick layer dominated by contorted fragments of pahoehoe lobes. Our field observations lead us to conclude that these breccias are formed by the disruption of an initial pahoehoe surface by a large flux of liquid lava within the flow. In the case of Laki, the lava flux was provided by surges in the erupted effusion rate. At Burfells it appears that the rapid flow came from the sudden breaching of the margins of a large ponded lava flow. Using the observations from Iceland, we have improved our earlier thermal modeling of the Martian flood lavas. We now conclude that these platy-ridged lava flows may have been quite thermally efficient, allowing the flow to extend for >100 km under a disrupted crust that was carried on top of the flow.

Using social norms theory to explain perceptions and sexual health behaviors of undergraduate college students: an exploratory study.

Abstract: The authors and associates conducted a social normsbased intervention targeting high-risk sexual behaviors among undergraduate students at 4 college campuses. Social norms theory predicts that widely held misperceptions may encourage risky behavior in a misguided attempt to conform to perceived norms and that information correcting these misperceptions will lead to a decrease in such behaviors. Students overestimated their peers' levels of sexual activity, numbers of partners, incidence of sexually transmitted infections, and rates of unintended pregnancies, but underestimated rates of condom use. Rates of HIV test taking, however, were accurately estimated. Although some components of sexual risk behaviors lend themselves well to social normsbased interventions, others, specifically inconsistent condom use and avoiding HIV tests, do not. Although no changes in reported beliefs or practices were apparent at the end of a 9-month intervention period, longer or modified interventions may be needed to make a ...

Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein

Abstract: Three new red-excitable monomeric fluorescent proteins obtained by structure-guided mutagenesis of mNeptune are described in this work. The authors show the use of one of them, mCardinal, to visualize the differentiation of myoblasts into myocytes in living mice.