Mitsubishi

About: Mitsubishi is a company organization based out in Tokyo, Japan. It is known for research contribution in the topics: Signal & Layer (electronics). The organization has 53115 authors who have published 54821 publications receiving 870150 citations. The organization is also known as: Mitsubishi Group of Companies & Mitsubishi Companies.

The R‐type pyocin of Pseudomonas aeruginosa is related to P2 phage, and the F‐type is related to lambda phage

Abstract: Pseudomonas aeruginosa produces three types of bacteriocins: R-, F- and S-type pyocins. The S-type pyocin is a colicin-like protein, whereas the R-type pyocin resembles a contractile but non-flexible tail structure of bacteriophage, and the F-type a flexible but non-contractile one. As genetically related phages exist for each type, these pyocins have been thought to be variations of defective phage. In the present study, the nucleotide sequence of R2 pyocin genes, along with those for F2 pyocin, which are located downstream of the R2 gene cluster on the chromosome of P. aeruginosa PAO1, was analysed in order to elucidate the relationship between the pyocins and bacteriophages. The results clearly demonstrated that the R-type pyocin is derived from a common ancestral origin with P2 phage and the F-type from lambda phage. This notion was supported by identification of a lysis gene cassette similar to those for bacteriophages. The gene organization of the R2 and F2 pyocin gene cluster, however, suggested that both pyocins are not simple defective phages, but are phage tails that have been evolutionarily specialized as bacteriocins. A systematic polymerase chain reaction (PCR) analysis of P. aeruginosa strains that produce various subtypes of R and F pyocins revealed that the genes for every subtype are located between trpE and trpG in the same or very similar gene organization as for R2 and F2 pyocins, but with alterations in genes that determine the receptor specificity.

A New Attenuation Relation for Strong Ground Motion in Japan Based on Recorded Data

Abstract: Following the 1995 Hyogo-ken Nanbu Kobe. Earthquake, the Japanese government, in an effort to prevent future earthquake disasters, installed networks consisting of a large number of strong-motion observation stations. Further, national seismic hazard maps were made available to the public on an Internet website in March 2005 by the Headquarters for Earthquake Research Promotion. However, these maps indicate only the local seismic intensity for Japan, as empirically con- verted from predicted peak velocity in consolidated soils. For various applications, other strong-motion indexes such as the response spectral acceleration are required. In this study, a database of whole Japanese strong ground motion records between 1963 and 2003 is established in order to identify a new standard attenuation relation for Japan, for response acceleration as well as peak value. It is usually very difficult to determine a suitable model form due to the large variability of strong-motion data and correlation among the model variables, because the strong coupling of variables in an attenuation model, and the statistical power of the data is often not large enough to determine the necessity of these parameters. Therefore, in this study, our model has only three variables: earthquake magnitude, shortest distance to the seismic fault plane, and focal depth. To improve predictions given by the model, site correction terms are adopted and additional terms for correcting regional anomalous seismic intensity with respect to the base model are determined. The good fit between the model and observed strong-motion records suggests that the new model is reasonably robust.

Three-dimensional structure of ribonuclease H from E. coli

Abstract: THE three-dimensional structure of RNase H from Escherichia coli was determined at 1.8 A resolution by X-ray crystallography. The enzyme was found to belong to the α + β class of structures, consisting of two distinct domains. The structure implies a possible region interacting with a DNA–RNA hybrid. The Mg2+-binding site essential for activity is located near a cluster of four acidic amino acids— one glutamic and three aspartic acid residues. These residues are completely conserved in the homology alignment of sequences of RNase H and reverse transcriptases from retro viruses and retrovirus-like entities1,2. The structural motif of β strands around the Mg2+-binding site has similarities to that in DNase I3–6.

Motor-driven power steering apparatus for automobiles

Abstract: A motor-driven type power steering apparatus for assisting a driver in manipulating a steering wheel of a motor vehicle includes a steering-wheel return state decision circuit for deciding whether the steering wheel is in the state to be returned to a center position, a control quantity generating and selecting circuit for generating control quantities corresponding to respective driving modes on the basis of a control quantity of the electric motor and selecting one of the control quantities in dependence on the result of the decision made as to the return state of the steering wheel, and a conversion circuit for converting the control quantities into PWM duty ratios, respectively In the normal steering operation, switching elements for energizing the motor are driven with the PWM duty ratio corresponding to a first driving mode, while upon return of the steering wheel to the center position, the switching elements are driven with the PWM duty ratio corresponding to a second driving mode A smooth steering torque control is realized with the steering-wheel return characteristic being improved