Deen Dayal Upadhyay Gorakhpur University

About: Deen Dayal Upadhyay Gorakhpur University is a education organization based out in Gorakhpur, Uttar Pradesh, India. It is known for research contribution in the topics: Thermal decomposition & Lymnaea acuminata. The organization has 1032 authors who have published 1591 publications receiving 21734 citations. The organization is also known as: Gorakhpur University.

Purification, characterisation and application of α‐l‐rhamnosidase from Penicillium citrinum MTCC‐8897

Abstract: Summary An α-l-rhamnosidase secreted by Penicillium citrinum MTCC-8897 has been purified to homogeneity from the culture filtrate of the fungal strain using ammonium sulphate precipitation and cation-exchange chromatography on carboxymethyl cellulose. The sodium dodecyl sulphate/polyacrylamide gel electrophoresis analysis of the purified enzyme gave a single protein band corresponding to the molecular mass 51.0 kDa. The native polyacrylamide gel electrophoresis also gave a single protein band confirming the enzyme purity. The Km and Vmax values of the enzyme for p-nitrophenyl α-l-rhamnopyranoside were 0.36 mm and 22.54 μmole min−1 mg−1, respectively, and kcat value was 17.1 s−1 giving kcat/Km value of 4.75 × 104 m−1 s−1. The pH and temperature optima of the enzyme were 7.0 and 60 °C, respectively. The purified enzyme liberated l-rhamnose from naringin, rutin, hesperidin and wine, indicating that it has biotechnological application potential for the preparation of l-rhamnose and other pharmaceutically important compounds from natural glycosides containing terminal α-l-rhamnose and also in the enhancement of wine aroma.

Computational study on the thermal decomposition and isomerization of the CH3OCF2O• radical

Abstract: The geometries of the reactant, products, and transition states involved in the decomposition pathways of the CH3OCF2O• radical formed during the photooxidation of CH3OCHF2 (HFE-152a) have been optimized and characterized at the DFT-B3LYP level of theory using the 6–311G(d,p) basis set. Single-point energy calculations have been made at the G2M (CC,MP2) level of theory. Out of the four prominent decomposition channels considered, the β-C–O bond scission is found to be the dominant path involving a barrier height of 9.78 kcal mol–1 (1 cal = 4.184 J). The thermal rate constant for the above decomposition pathway is evaluated using canonical transition state theory (CTST) and was found to be 5.27 × 104 s–1 at 298 K and 1 atm (1 atm = 101.325 kPa).

Plant essential oils: a substitute for conventional insecticides against Tribolium species (Coleoptera: Tenebrionidae)-achievements and challenges

Abstract: The literature on efficacy of plant essential oils and their constituents for the management of Tribolium spp. (T. castaneum and T. confusum), instigating infestation of a variety of stored grains ...