Deen Dayal Upadhyay Gorakhpur University

About: Deen Dayal Upadhyay Gorakhpur University is a education organization based out in Gorakhpur, Uttar Pradesh, India. It is known for research contribution in the topics: Thermal decomposition & Lymnaea acuminata. The organization has 1032 authors who have published 1591 publications receiving 21734 citations. The organization is also known as: Gorakhpur University.

Purification and characterization of polygalacturonase from Aspergillus fumigatus MTCC 2584 and elucidating its application in retting of Crotalaria juncea fiber.

Abstract: Polygalacturonases represents an important member of pectinases group of enzymes with diverse industrial applications and is widely distributed among fungi, bacteria, yeasts, plants and some plant parasitic nematodes. An endo-polygalacturonase from a new fungal source Aspergillus fumigatus MTCC 2584 was produced under solid-state fermentation conditions and was purified simply by acetone precipitation and gel-filtration chromatography technique. The approximate molecular weight of the purified PG was found to be 43.0 kDa as revealed by SDS-PAGE. The pH optimum of the purified enzyme was found to be 10.0 and was stable in the pH range of 7–10. The optimum temperature of purified PG was found to be 30 °C. The Km and Kcat of the purified enzyme were 2.4 mg/ml and 44 s−1, respectively, and the metal ions Cu2+ and K+ were found to enhance the enzyme activity while Ag+, Ca2+ and Hg2+ were inhibitory in nature. Based on its alkaline nature, the potential of purified PG in retting of natural fiber Crotalaria juncea was elucidated in the absence of EDTA. This is probably the first report of alkaline PG from Aspergillus fumigatus.

Isolation of repellent ingredients from Lantana camara (Verbenaceae) flowers and their repellency against Aedes mosquitoes

Abstract: : The repellent properties of different fractions isolated from Lantana camara flowers by using steam distillation, solvent partition and chromatographic methods were evaluated against Aedes mosquitoes. Maximum protection time of one fraction eluted by chloroform from a silica gel column was 3.45 h. One application of this fraction gave 100% protection for 2 h and may protect 75.8% at 7 h (t = 7.00, P < 0.001) against the bites of Aedes mosquitoes. Further purification of the most efficient fraction into pure compounds did not result in any increase in repellency.

Synthesis, spectral and antimicrobial studies of diorganotin(IV)3(2'-hydroxyphenyl)-5-(4-substituted phenyl) pyrazolinates

Abstract: Diorganotin(IV) dipyrazolinates of the type R 2 Sn(C 15 H 12 N 2 OX) 2 [where C 15 H 12 N 2 OX = 3(2'-Hydroxyphenyl)-5(4-X-phenyl)pyrazoline {where X = H (a); CH 3 (b); OCH 3 (c); Cl (d) and R = Me, Pr" and Ph}] have been synthesized by the reaction of R 2 SnCl 2 with sodium salt of pyrazolines in 1:2 molar ratio, in anhydrous benzene. These newly synthesized derivatives have been characterized by elemental analysis (C, H, N, Cl and Sn), molecular weight measurement as well as spectral [IR and multinuclear NMR ( 1 H, 13 C and 119 Sn)] studies. The bidentate behaviour of the pyrazoline ligands was confirmed by IR, 1 H and 13 C NMR spectral data. A distorted trans-octahedral structure around tin(IV) atom for R 2 Sn(C 15 H 12 N 2 OX) 2 has been suggested. The free pyrazoline and diorganotin(IV) dipyrazolinates have also been screened for their antibacterial and antifungal activities. Some diorganotin(IV) dipyrazolinates exhibit higher antibacterial and antifungal effect than free ligand and some of the antibiotics.

Analysis of genetic diversity in cowpea ( Vigna unguiculata L.Walp.) cultivars with random amplified polymorphic DNA markers

Abstract: The genetic diversity among ten Indian cultivars of cowpea was analyzed using 18 sets of RAPD markers. A total of 181 bands with an average of 15 bands per primer were obtained. Out of 181 bands, 148 showed polymorphism (81.7%). The variation in genetic diversity among these cultivars ranged from 0.1742 to 0.4054. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA with high bootstrap values revealed two distinct clusters I and II comprised of two and seven cultivars, respectively. Cluster II was further differentiated into various subclusters. Cultivar IC-9883 was found to be unique based on its altogether distinct position in the dendrogram and two-dimensional space projections.