Agilent Technologies

About: Agilent Technologies is a company organization based out in Santa Clara, California, United States. It is known for research contribution in the topics: Signal & Mass spectrometry. The organization has 7398 authors who have published 11518 publications receiving 262410 citations. The organization is also known as: Agilent Technologies, Inc..

Analysis and design of broad-band single-layer rectangular U-slot microstrip patch antennas

Abstract: A wide operating bandwidth for a single-layer coaxially fed rectangular microstrip patch antenna can be obtained by cutting a U-shaped slot on the patch. This antenna structure has recently been found experimentally to provide impedance bandwidths of 10%-40%, even with nonair substrates. However, design rules for this antenna have not yet been presented. This paper develops principle design procedures through examination of the structure's multiple resonant frequencies as well as the radiation and impedance properties of different antenna geometries. The approximate design rules are derived by analysis of former experiments, method of moments (MoM) simulations, and measurement results. Simulations and measurements of several antennas designed using these new rules are presented and directions for further study are discussed.

A HUPO test sample study reveals common problems in mass spectrometry–based proteomics

Abstract: We performed a test sample study to try to identify errors leading to irreproducibility, including incompleteness of peptide sampling, in liquid chromatography-mass spectrometry-based proteomics. We distributed an equimolar test sample, comprising 20 highly purified recombinant human proteins, to 27 laboratories. Each protein contained one or more unique tryptic peptides of 1,250 Da to test for ion selection and sampling in the mass spectrometer. Of the 27 labs, members of only 7 labs initially reported all 20 proteins correctly, and members of only 1 lab reported all tryptic peptides of 1,250 Da. Centralized analysis of the raw data, however, revealed that all 20 proteins and most of the 1,250 Da peptides had been detected in all 27 labs. Our centralized analysis determined missed identifications (false negatives), environmental contamination, database matching and curation of protein identifications as sources of problems. Improved search engines and databases are needed for mass spectrometry-based proteomics.

Navigation technique for detecting movement of navigation sensors relative to an object

Abstract: A scanning device and method for forming a scanned electronic image include using navigation information that is acquired along with image data, and then rectifying the image data based upon the navigation and image information. The navigation information is obtained in frames. The differences between consecutive frames are detected and accumulated, and this accumulated displacement value is representative of a position of the scanning device relative to a reference. The image data is then positioned-tagged using the position data obtained from the accumulated displacement value. To avoid the accumulation of errors, the accumulated displacement value obtained from consecutive frames is updated by comparing a current frame with a much earlier frame stored in memory and using the resulting difference as the displacement from the earlier frame. These larger displacement steps are then accumulated to determine the relative position of the scanning device.

A Permanent Mesoporous Organic Cage with an Exceptionally High Surface Area

Abstract: Recently, porous organic cage crystals have become a real alternative to extended framework materials with high specific surface areas in the desolvated state. Although major progress in this area has been made, the resulting porous compounds are restricted to the microporous regime, owing to the relatively small molecular sizes of the cages, or the collapse of larger structures upon desolvation. Herein, we present the synthesis of a shape-persistent cage compound by the reversible formation of 24 boronic ester units of 12 triptycene tetraol molecules and 8 triboronic acid molecules. The cage compound bears a cavity of a minimum inner diameter of 2.6 nm and a maximum inner diameter of 3.1 nm, as determined by single-crystal X-ray analysis. The porous molecular crystals could be activated for gas sorption by removing enclathrated solvent molecules, resulting in a mesoporous material with a very high specific surface area of 3758 m2 g−1 and a pore diameter of 2.3 nm, as measured by nitrogen gas sorption.

Direct and sensitive miRNA profiling from low-input total RNA.

Abstract: We have developed a sensitive, accurate, and multiplexed microRNA (miRNA) profiling assay that is based on a highly efficient labeling method and novel microarray probe design. The probes provide both sequence and size discrimination, yielding in most cases highly specific detection of closely related mature miRNAs. Using a simple, single-vial experimental protocol, 120 ng of total RNA is directly labeled using Cy3 or Cy5, without fractionation or amplification, to produce precise and accurate measurements that span a linear dynamic range from 0.2 amol to 2 fmol of input miRNA. The results can provide quantitative estimates of the miRNA content for the tissues studied. The assay is also suitable for use with formalin-fixed paraffin-embedded clinical samples. Our method allows rapid design and validation of probes for simultaneous quantitative measurements of all human miRNA sequences in the public databases and to new miRNA sequences as they are reported.