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Open AccessJournal ArticleDOI

A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality

Tanya L. Daigle, +40 more
- 12 Jul 2018 - 
- Vol. 174, Iss: 2, pp 465-480
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TLDR
A large set of driver and reporter transgenic mouse lines are reported, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools.
About: 
This article is published in Cell.The article was published on 2018-07-12 and is currently open access. It has received 511 citations till now.

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Citations
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza, +462 more
- 01 Oct 2019 - 
TL;DR: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community providing the theory and key practical aspects offlow cytometry enabling immunologists to avoid the common errors that often undermine immunological data.
Journal ArticleDOI

The Allen Mouse Brain Common Coordinate Framework: A 3D Reference Atlas.

Quanxin Wang, +24 more
- 14 May 2020 - 
TL;DR: This work constructed an average template brain at 10 μm voxel resolution by interpolating high resolution in-plane serial two-photon tomography images with 100 μm z-sampling from 1,675 young adult C57BL/6J mice and parcellated the entire brain directly in 3D.
Journal ArticleDOI

Hierarchical organization of cortical and thalamic connectivity

Julie A. Harris, +42 more
- 30 Oct 2019 - 
TL;DR: Using mouse lines in which subsets of neurons are genetically labelled, the authors provide generalized anatomical rules for connections within and between the cortex and thalamus, showing that cell-class-specific connections are organized in a shallow hierarchy within the mouse corticothalamic network.
Journal ArticleDOI

Single-nucleus and single-cell transcriptomes compared in matched cortical cell types.

Trygve E. Bakken, +27 more
- 26 Dec 2018 - 
TL;DR: It is demonstrated that closely related neuronal cell types can be similarly discriminated with both methods if intronic sequences are included in snRNA-seq analysis, and the high information content of nuclear RNA for characterization of cellular diversity in brain tissues is illustrated.
Journal ArticleDOI

Classification of electrophysiological and morphological neuron types in the mouse visual cortex.

Nathan W. Gouwens, +94 more
- 17 Jun 2019 - 
TL;DR: A single-cell characterization pipeline is established using standardized patch-clamp recordings in brain slices and biocytin-based neuronal reconstructions to establish a morpho-electrical taxonomy of cell types for the mouse visual cortex via unsupervised clustering analysis of multiple quantitative features.
References
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Journal ArticleDOI

limma powers differential expression analyses for RNA-sequencing and microarray studies

Matthew E. Ritchie, +7 more
- 20 Apr 2015 - 
TL;DR: The philosophy and design of the limma package is reviewed, summarizing both new and historical features, with an emphasis on recent enhancements and features that have not been previously described.
Journal ArticleDOI

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong, +15 more
- 15 Feb 2013 - 
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong, +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI

A robust and high-throughput Cre reporting and characterization system for the whole mouse brain

Linda Madisen, +11 more
- 01 Jan 2010 - 
TL;DR: A set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra is generated and enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in mapping neuronal circuitry, imaging and tracking specific cell populations in vivo.
Journal ArticleDOI

A mesoscale connectome of the mouse brain

Seung Wook Oh, +33 more
- 10 Apr 2014 - 
TL;DR: A brain-wide, cellular-level, mesoscale connectome for the mouse, using enhanced green fluorescent protein-expressing adeno-associated viral vectors to trace axonal projections from defined regions and cell types, and high-throughput serial two-photon tomography to image the EGFP-labelled axons throughout the brain.
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