Philip L. Lorenzi

TL;DR: The sensitivity and accuracy of Gln, Glu, and pGlu quantitation by electrospray ionization-based mass spectrometry can be improved dramatically by using chromatographic conditions that adequately separate the three metabolites, isotopic internal standards to correct for in-source pGLU formation, and user-optimized fragmentor voltage for acquisition of the MS spectra.

TL;DR: The substrate specificity suggests that the substrate-binding pocket of BPHL has a hydrophobic acyl binding site which can accommodate the positively charged alpha-amino group, while having an alcohol leaving group binding site that can accommodate nucleoside analogues with a relatively generous spatial allowance.

TL;DR: Plasma asparagine depletion by RBC-entrapped L-asparaginase in selected patients having no low or no ASNS may be a promising therapeutic approach for pancreatic cancer.

TL;DR: Levels of cellular E-cad expression are associated with levels of cell-cell adhesion and response to drug treatment, and Predictions of E- cad regulation based on the above factors were tested and verified by demethylation studies.