Carl G. Maki

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: In this study, inhibitors of the 26S proteasome have been used to further explore the role of ubiquitin proteolysis in regulating p53 turnover and indicate that ubiquitIn-p53 conjugates were detected in untreated as well as gamma-irradiated cells, indicating that Ubiquitin-dependent proteolytic plays a role in the normal turnover of p53.

TL;DR: The results indicate that MDM2-mediated ubiquitination, or other activities associated with the RING-finger domain, can stimulate the export of p53 to the cytoplasm through the activity of the p53 NES.

TL;DR: It is demonstrated that UV and gamma radiation have different effects on ubiquitinated p53 and suggested that the UV-induced stabilization of p53 results from a loss of p 53 ubiquitination, and that the radiation effects on p53 are specific.

TL;DR: Results suggest that the hypoxic induction of p53 is attributable to the down-regulation of MDM2 protein levels and uncoupling of p 53 from its interaction with the E6/E6AP complex.