Takahiko Akematsu

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: Author(s): Klionsky, DJ; Abdelmohsen, K; Abe, A; Abedin, MJ; Abeliovich, H; A Frozena, AA; Adachi, H, Adeli, K, Adhihetty, PJ; Adler, SG; Agam, G; Agarwal, R; Aghi, MK; Agnello, M; Agostinis, P; Aguilar, PV; Aguirre-Ghis

TL;DR: In this paper, the first direct observation of programmed post-meiotic DNA double-strand breaks (PM-DSBs) via the electrophoretic separation of DSB-generated DNA fragments in the ciliate Tetrahymena thermophila was reported.

TL;DR: Knocking out or reducing the expression of APRT1 by RNA interference (RNAi) did not affect robust cell growth, which indicates that adenine salvage is redundant or that de novo synthesis pathways provide sufficient adenosine monophosphate for viability.

TL;DR: It is shown that a transmembrane protein, Semi1, is crucial for attachment in Tetrahymena thermophila, indicating that attachment of a nucleus to the junction is not a precondition for selection but follows the selection process.