scispace - formally typeset
R

Ryuichiro Atarashi

Researcher at University of Miyazaki

Publications -  68
Citations -  9028

Ryuichiro Atarashi is an academic researcher from University of Miyazaki. The author has contributed to research in topics: PrPSc Proteins & Scrapie. The author has an hindex of 28, co-authored 64 publications receiving 8141 citations. Previous affiliations of Ryuichiro Atarashi include Nagasaki University & National Institutes of Health.

Papers
More filters
Journal ArticleDOI

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky, +2522 more
- 21 Jan 2016 - 
TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
Journal ArticleDOI

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion

TL;DR: A new PrPSc amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg ofPrPSc in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate, and indicates the promising enhanced diagnostic capacity of RT- QUIC in the antemortem evaluation of suspected CJD.
Journal ArticleDOI

Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein.

TL;DR: A much faster seeded polymerization method (rPrP-PMCA) is described which detects ≥50 ag of hamster PrPSc (≈0.003 lethal dose) within 2–3 d and should facilitate the development of rapid, ultrasensitive prion assays and diagnostic tests, in addition to aiding fundamental studies of structure and mechanism of PrP Sc formation.
Journal ArticleDOI

Simplified ultrasensitive prion detection by recombinant PrP conversion with shaking.

TL;DR: A new prion assay, abbreviated QUIC for quaking-induced conversion, which uses rPrP-sen as a substrate and automated tube shaking rather than sonication is developed, which can detect about one lethal prion dose within a day, and is faster and simpler than previous described PMCA6 and rPrp-PMCA5 assays.