Jiri Stulik

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: Five approaches presented here resulted in the detection of disease‐associated proteins, including Calgranulin B was upregulated in colorectal cancer, and hepatoma‐derived aldose reductase‐like protein was reexpressed in a rat model during hepatocarcinogenesis.

TL;DR: The current knowledge of the TPR-containing proteins involved in virulence mechanisms of bacterial pathogens are summarized while highlighting the importance of TPR motifs for the proper functioning of class II chaperones of a type III secretion system in the pathogenesis of Yersinia, Pseudomonas, and Shigella.

TL;DR: It is demonstrated here that ClpB of F. tularensis live vaccine strain (LVS) is important for resistance to cellular stress and promoter analysis shows that the transcriptional start is preceded by a σ32‐like promoter sequence and expression of clpB is induced by heat shock, indicating that expression of ClpP is dependent on the heat‐shock response mediated by ρ32, the only alternative σ‐factor present in Francisella.

TL;DR: Comprehensive mapping of immunoreactive antigens using the attenuated live vaccine strain of F. tularensis LVS originating from the European virulent strain finds that 60 kDa and 10 kDa chaperonins that occurred in several charge and mass variants, predominated.