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Journal ArticleDOI

Proteasome inhibitors: valuable new tools for cell biologists.

Do Hee Lee, +1 more
- 01 Oct 1998 - 
- Vol. 8, Iss: 10, pp 397-403
TLDR
The actions of selective proteasome inhibitors are described, how they can be used to investigate cellular responses, the functions of the proteasomes demonstrated by such studies and their potential applications in the future.
About: 
This article is published in Trends in Cell Biology.The article was published on 1998-10-01. It has received 1459 citations till now. The article focuses on the topics: Endoplasmic-reticulum-associated protein degradation & Protein degradation.

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Citations
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Journal ArticleDOI

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky, +2522 more
- 21 Jan 2016 - 
TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
Journal ArticleDOI

Guidelines for the use and interpretation of assays for monitoring autophagy

Daniel J. Klionsky, +1287 more
- 01 Apr 2012 - 
TL;DR: These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
Journal ArticleDOI

Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

Daniel J. Klionsky, +235 more
- 16 Feb 2008 - 
TL;DR: A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.
Journal ArticleDOI

The 26S Proteasome: A Molecular Machine Designed for Controlled Proteolysis

D. Voges, +2 more
- 01 Jan 1999 - 
TL;DR: In eukaryotic cells, most proteins in the cytosol and nucleus are degraded via the ubiquitin-proteasome pathway, and the 26S proteasome is a 2-MDa molecular machine built from approximately 31 different subunits, which catalyzes protein degradation.
Journal ArticleDOI

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Daniel J. Klionsky, +2983 more
- 08 Feb 2021 - 
TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.
References
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Journal ArticleDOI

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

Kenneth L. Rock, +7 more
- 09 Sep 1994 - 
TL;DR: Peptide aldehydes that inhibit major peptidase activities of the 20S and 26S proteasomes are shown to reduce the degradation of protein and ubiquitinated protein substrates by 26S particles.
Journal ArticleDOI

Structure and Functions of the 20S and 26S Proteasomes

Olivier Coux, +2 more
- 01 Jan 1996 - 
TL;DR: Major advances have been achieved recently in knowledge about the molecular organization of the 20S and 19S particles, their subunits, the proteasome's role in MHC-class 1 antigen presentation, and regulators of its activities.
Journal ArticleDOI

Structure of 20S proteasome from yeast at 2.4 A resolution.

Michael Groll, +6 more
- 03 Apr 1997 - 
TL;DR: Two β-type subunits are processed to an intermediate form, indicating that an additional nonspecific endopeptidase activity may exist which is important for peptide hydrolysis and for the generation of ligands for class I molecules of the major histocompatibility complex.
Journal ArticleDOI

The ubiquitinproteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB

Vito J. Palombella, +3 more
- 09 Sep 1994 - 
TL;DR: The ubiquitin-proteasome pathway has been shown to play an essential role in two proteolytic processes required for activation of the transcription factor NF-κB as discussed by the authors.
Journal ArticleDOI

The ubiquitin-proteasome proteolytic pathway

Aaron Ciechanover
- 07 Oct 1994 - 
TL;DR: Two studies clearly demonstrate that the ubiquitin-proteasome system is involved not only in complete destruction of its protein substrates, but also in limited proteolysis and posttranslational processing in which biologically active peptides or fragments are generated.
Related Papers (5)

The Ubiquitin System

Avram Hershko, +1 more
- 01 Jan 1998 - 

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

Kenneth L. Rock, +7 more
- 09 Sep 1994 - 

Structure and Functions of the 20S and 26S Proteasomes

Olivier Coux, +2 more
- 01 Jan 1996 - 

The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction

Michael H. Glickman, +1 more
- 01 Apr 2002 - 

The 26S Proteasome: A Molecular Machine Designed for Controlled Proteolysis

D. Voges, +2 more
- 01 Jan 1999 -